Beta-Lactamase DataBase (BLDB): Structure and Function
Beta-Lactamase DataBase
Beta-Lactamase DataBase - Structure and Function Home Enzymes Structures Mutants Kinetics BLAST
Beta-Lactamase DataBase

Kinetic parameters (work in progress)

Ambler Class Enzyme (Mutations) PubMedID
Benzylpenicillin
(BPN)
Penicillin G
(PCG)
Amoxicillin
(AMO)
Ampicillin
(AMP)
Oxacillin
(OXA)
Cloxacillin
(CLX)
Carbenicillin
(CRB)
Methicillin
(MTL)
Ticarcillin
(TIC)
Temocillin
(TEM)
Piperacillin
(PIP)
Azlocillin
(AZL)
Mezlocillin
(MZL)
Cefalothin
(CFT)
Cefuroxime
(CFU)
Cefotaxime
(CFX)
Ceftazidime
(CFZ)
Cefepime
(CFP)
Cefpirome
(CFR)
Cefoxitin
(CFN)
Cephaloridin
(CFD)
Cefazolin
(CEF)
Cefazolin (V0)
(CF0)
Cefazolin (Vss)
(CFS)
Cefsulodin
(CFI)
Aztreonam
(AZT)
Imipenem
(IMI)
Meropenem
(MER)
Ertapenem
(ERT)
Panipenem
(PAN)
Faropenem
(FAR)
Doripenem
(DOR)
Nitrocefin
(NIT)
Clavulanic acid
(CLA)
CENTA
(CEN)
A GES-1
22908160
Show profile:
30
120
250


13
180
72










7
1000
7
0.002
4000
0.00005
8
900
9




900
5300
170


370
9000
40
110
40000
27.5
25
30000
0.8


0.08
60
1.3
540
4600
120








4.8
4000
1.2
0.007
1.5
5
0.0007
0.1
7
0.003
0.4
7.5






380
340
1100




A GES-5
22908160
Show profile:
110
430
255


13
50
260










5
1600
3
0.003
4500
0.00007
160
1000
160




900
9000
100


36
25000
1.4
7
50000
0.14
8
90000
0.09


4.5
860
5
725
5000
145








0.06
1800
0.03
0.7
4.6
150
0.06
1
60
0.09
1.5
60






260
550
470




A GES-11
22908160
Show profile:
4
37
110


7
37
190










2.6
37
70
0.015
550
0.03
27
120
255




740
4000
185


1600
5500
290
990
38000
26
9
930
10


0.02
13
1.5
455
3600
130








53
3200
17
0.015
1
15
0.0035
0.1
35
0.002
0.2
10






320
530
600




A GES-12
22908160
Show profile:
16
60
270


16
73
220










4.6
214
21
0.16
1000
0.16
96
300
320




80
5300
15


586
9800
60
264
5350
50
8
1300
6


0.03
10
3
75
640
120








25
740
34
0.02
1
20
0.008
0.06
130
0.007
0.07
100






72
215
330




A GES-14
22908160
Show profile:
23
300
77


7
130
54










0.2
32
6
0.02
400
0.05
60
750
80




1100
6000
180


14
7900
1.8
2.5
20000
0.125
0.4
600
0.7


0.35
220
1.6
150
4400
34








3.5
5000
0.7
0.09
2.9
31
0.01
0.2
50
0.01
0.3
33






56
135
415




A GES-18
23114760
Show profile:
70
400
175


7
40
175










5
1000
5
0.004
3500
0.001
75
700
100




500
6000
80


>20
>20000
1
>2.5
>20000
0.125
>1
>20000
0.05


3.4
1000
3
360
4600
70








0.08
3000
0.02
0.4
3.5
110
0.04
1
40
0.06
1.3
40






100
330
300




A KPC-2
18625772
Show profile:
45
44
1000




20
29
700








ND
NH
0


40
51
800




106
141
750
66
160
410
66
174
380
ND
NH
0


12
251
500












3
35
900
20
88
230
















A KPC-2
20662006
Show profile:




















51
15
3300




44
14
3100


142
207
700




















19
19
1000








0.73
5.5
130
52
6
8500




A KPC-2
22687511
Show profile:




















65
12
5400




223
38
5900


140
190
700




















21
21
1000










130
8
16000




A KPC-2 W105F
20662006
Show profile:




















112
24
4700




219
27
8100


102
200
500




















9
15
600










96
11
8700




A KPC-2 W105N
20662006
Show profile:




















89
34
2600




219
47
4700


ND
>200
0




















3.1
33
90










104
24
4300




A KPC-2 W105L
20662006
Show profile:




















ND
>100
0




175
177
1000


ND
>200
0




















1.5
24
60








0.75
4.8
210
97
61
1600




A KPC-2 W105V
20662006
Show profile:




















50
37
1400




94
127
1500


ND
>200
0




















5.5
83
70










103
62
1700




A KPC-2 T237S
20421396
Show profile:




















42
6
7000




65
18
3600


43
168
260




















21
21
1000










50
5
10000




A KPC-2 T237A
20421396
Show profile:




















42
12
3500




26
30
900


<0.1
ND
ND




















6.8
31
220










191
15
13000




A KPC-2 R220H
22687511
Show profile:




















68
13
5200




206
108
1900


ND
ND
0




















9
11
800










260
16
16000




A KPC-2 R220A
22687511
Show profile:




















193
164
1200




379
207
1800


ND
ND
0




















4
18
200










327
54
6100




A KPC-2 R220M
22687511
Show profile:




















85
35
2400




156
98
1600


ND
ND
0




















10
28
400










724
94
7700




A KPC-2 R220K
22687511
Show profile:




















40
23
1700




88
45
2000


ND
ND
0




















7
5
1400










120
10
12000




A KPC-2 E276A
22687511
Show profile:




















59
12
4800




157
24
6500


118
278
400




















11
12
900










97
4.9
19000




A KPC-2 E276D
22687511
Show profile:




















38
5.2
7200




139
12
1100


100
253
500




















13
10
1300










77
4.1
19000




B1 IMP-1
19770275
Show profile:


320
520
620


950
200
4800












ND
ND
0




48
21
2400
8
37
220
1.3
4
350
8
44
180
7
11
660


16
8
2000










>0.01
>1000
0
46
39
1200
50
10
120














B1 NDM-1
19770275
Show profile:


11
16
680


15
22
660












14
12
1170




4
10
400
5
8
610
6
10
580
5
181
30
13
77
170


1
49
20










ND
ND
0
20
94
210
12
49
250










ND
ND
0


B1 VIM-2
19770275
Show profile:


56
49
1140
















33
72
450




57
44
1280
12
22
550
28
32
860
89
98
900
5
184
30


3
24
120










<0.5
ND
0
10
10
990
1
5
280














B3 BJP-1
20696874
Show profile:







670
19









ND
0



700
67





230
580





>700
4.3



















260
60

190
830














B3 FEZ-1
20696874
Show profile:







>5000
11









>5000
13



4200
12





120
2500





>1000
4



















>1000
200

85
500














B3 GOB-1
20696874
Show profile:

















150
520



170
1700





24
670





71
760



















60
660

5
5300














B3 L1
20696874
Show profile:







40
4400









140
2700



20
7000





8.9
9200





145
200



















90
730

10
4500














D OXA-1
20086146
Show profile:






357
64
6000
577
31
19000






























16
80
210






















94
9
10000




D OXA-2
16870757
Show profile:
90
4
22000




110
25
4400
900
360
2500
















3.4
7
490




0.02
12
1.7




































D OXA-20
9687410
Show profile:


26
4.4
5900


80
33
2400
116
329
350
70
788
88




4
147
30








13
5
2600




ND
ND
0




ND
ND
0
20
69
300








6
69
90


















D OXA-22
10898703
Show profile:
0.1
<2
>40


0.1
6
16


ND
ND
0
0.7
5
140




0.1
9
10


ND
ND
0




0.9
3
330
0.05
5
10
ND
ND
0
ND
ND
0




0.05
10
4
5.5
10
550








ND
ND
0


















D OXA-29
11709332
Show profile:


65
10
6500


107
16
6700
34
4.4
7700


13
16
810
27
41
660




31
39
790






>0.19
>250
0.79
0.17
128
1.3
>0.24
>500
0.49








11
30
370






0.21
210
1
NH
NH
0
NH
NH
0








96
1800
19000




D OXA-32
11796380
Show profile:
3.5
45
80


1.5
220
70




2.5
110
20




1
60
15


3
155
20




3
60
50


ND
ND
0
H
>3000
H


ND
ND
0


2
360
6








ND
ND
0


















D OXA-40
12499201
Show profile:
5
23
220




5
220
20
2
876
3






1
60
20


1
23
50




3
72
50


ND
NH
0
20
2500
10
ND
NH
0
ND
NH
0


5
1000
5








ND
NH
0
0.1
6.5
15
ND
NH
0














D OXA-46
15855521
Show profile:


18
48
380


6
20
300
300
320
940


85 ; 5
730 ; 545
1200 ; 9.2




NH
>1000
0


11
46
240
12
30
400
8
23
350
NH
>1000
0
NH
>1000
0
NH
>1000
0
NH
>1000
0








17
23
740
7
40
170
NH
>1000
0
NH
>1000
0












20
18
1100




D OXA-48
23305656
Show profile:
245
40
6100




340
5200
65
25
30
850






45
55
820
0.3
45
6.5
75
410
180




3
20
150


10
190
60
4
5100
1
1
160
6














ND
NH
0
2
14
145
0.1
200
0.5
NA
NA
NA












D OXA-48
19477418
Show profile:






955
395
2400
130
95
1400








0.3
45
6.6






44
195
230


>9
>900
10
ND
NH
0
>0.6
>550
1.1


>0.05
>200
0.26












4.8
13
370
0.07
11
6.2
0.13
100
1.3
1.4
14
100
0.038
13
2.9


940
120
7700




D OXA-54
14693565
Show profile:
120
60
2000




540
4300
125
35
75
500






30
240
125


20
240
85




3
230
15


15
1600
10
ND
NH
0
1
180
5
4
110
35


1
45
20








ND
NH
0
1
4
250
0.1
125
1














D OXA-57
15793160
Show profile:


0.4
29.5
13.6


0.54
71.9
7.54
1.23
40.7
30.2
0.51
18.7
17.9








2.11
21.3
99




0.28
163
1.73












13
97.9
133






















63
17.9
3510




D OXA-57 D170N
15793160
Show profile:


0.61
56.3
10.7


2.12
38.9
54.4
2.37
37.2
63.7
1.12
166
6.69








5.7
22.1
258




0.86
100
8.59




































79.5
50.4
1580




D OXA-57 K232N
15793160
Show profile:


0.75
46.6
16


1.42
157
8.98
3.07
194
15.7
1.04
180
5.77








4.31
9.48
455




0.65
55.4
11.7




































29.7
13.2
2240




D OXA-85b
16870757
Show profile:
830
10
83000




45
76
570
780
36
21000






>10
>250
40








4.1
47
87
>1.8
>400
4.5
ND
NH
0
ND
NH
0
ND
NH
0
































0.42
23
18
D OXA-114a
18362192
Show profile:
0.1
10
10






<0.01
ND
0






0.1
40
2.5


0.5
1
100




0.5
15
30


<0.01
ND
0
<0.01
ND
0




<0.01
ND
0












>0.1
>2000
0.05
















D OXA-163
21422200
Show profile:
15
40
380




25
320
80








1
320
3


8
70
110




3
80
40


30
850
35
200
>2000
1000
2
350
6














0.5
230
2
0.03
530
0.05
0.07
2200
0.03
0.01
150
0.1












D OXA-181
23305656
Show profile:
445
125
3560




200
130
1500
70
80
875






65
100
650
0.2
85
2.5
75
210
350




3
125
25


4
75
55
ND
NH
0
15
1120
15














ND
NH
0
4
40
100
0.1
70
1
0.1
85
1












D OXA-232
23305656
Show profile:
510
60
8500




380
175
2200
240
100
2400






480
135
3600
0.04
70
0.5
380
540
700




7
125
50


6
195
30
ND
NH
0
13
1200
10














ND
NH
0
1
35
30
0.1
125
1
0.1
100
1












NOTE: In each cell, the first value is kcat (s-1), the second value is Km (µM) and the third value is kcat/Km (s-1·mM-1). When two values are present on the same line, the first correspond to V0 and the second to Vss.

Abbreviations: ND - not determinable; NH – no hydrolysis detected; NA – not available.

Statistics (number of kinetics): Overall (47); class A (21); subclass B1 (3); subclass B2 (0); subclass B3 (4); class C (0); class D (19).

Last updated: November 06, 2021.

If you use BLDB please cite: Naas, T.; Oueslati, S.; Bonnin, R. A.; Dabos, M. L.; Zavala, A.; Dortet, L.; Retailleau, P.; Iorga, B. I., Beta-Lactamase DataBase (BLDB) – Structure and Function. J. Enzyme Inhib. Med. Chem. 2017, 32, 917-919.

The development of the BLDB database is funded in part by the JPIAMR transnational project DesInMBL, the Région Ile-de-France (DIM Malinf) and the Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT).

Contact: contact@bldb.eu